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KMID : 0613820140240020154
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Journal of Life Science
2014 Volume.24 No. 2 p.154 ~ p.160
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The Complex Salmonella typhimurium Ghost Cells Play an Additional Role as an Immune Adjuvant
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Ha Yeon-Jo
Kim Seung-Tae
Kang Ho-Young
Gal Sang-Wan
Kim Sam-Woong
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Abstract
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Ghost cells have been recognized as eliciting humoral and cell-mediated immune responses and have also been predicted to play a role as an immune adjuvant. In this study, we used the intramuscular (IM) route to inject BALB/c mice with four vaccine groups constructed from Salmonella typhimurium ghost (STG) cells originating from different virulent strains and complex STG groups instead of heat-labile toxin (LT)-B, a type of adjuvant. Although the complex STG groups exhibited a response after a short delay, the groups showed final total IgG levels similar to those of the LT-B group, which encodes LT-B from pMMP300. The IgG1 response to the χ3339 group was the highest response at 6 weeks, whereas IgG2a responses to the χ3339 and JOL389 groups were higher at 6 and 8 weeks compared to those of the LT-B group. The response of vaginal sIgA to the LT-B group was generally higher than that of the other groups, whereas fecal sIgA to the LT-B group exhibited lower responses. Protection to virulent S. typhimurium in all groups was above 80%, which was similar to the LT-B group. Taken together, we suggest that STG complex groups can be used as an immune adjuvant instead of LT-B.
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KEYWORD
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Antibody, ghost, intramuscular, labile toxin (LT)-B, Salmonella
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